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Animal Cell Culture
Titre : Animal Cell Culture Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : 2013 Collection : Importance : xiv, 713 p. Présentation : ill. ISBN/ISSN/EAN : 978-1-59259-492-4 Langues : Anglais Tags : Cell culture -- Tissue culture -- Technique Index. décimale : Résumé : For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques for establishing such a facility. Much had to be done by trial and error or gleaned from originalpapers. Consequently, I felt that a methods book covering a wide variety of techniques from basic culture to the most sophisticated cell analysis would be a very valuable addition to the scientific literature. In the interim, several useful books (listed in Chapter I of this volume) did appear, but none entirely fitted the bill and some are now somewhat dated. Then, in 1984, the first of the Methods in Molecular Biology volumes from Humana Press was published with its step-by-step recipe approach. This format appealed to me, and so I c- tacted John Walker, the series editor, about including cell culture in this series. The result was that we embarked upon a single volume covering both plant and animal cell culture. Such was the richness of the material that this project soon divided itself into separate volumes on animal cell (Volume 5) and plant cell (Volume 6) culture. In this volume (Volume 5), therefore, we have aimed to describe a variety of basic techniques and culture conditions for a range of cell types.
Contents
Basic cell culture / Jeffrey W. Pollard -- Establishment, maintenance, and cloning of human primary cell strains / Gareth E. Jones -- Aging of cultured human skin fibroblasts / Calvin B. Harley -- Separation and maintenance of primary T and B lymphocytes / Derek Kinchington, Eleanor Berrie -- Establishment of lymphoblastoid cell lines / A. Doyle -- Scale-up of suspension and anchorage-dependent animal cells / J. Bryan Griffiths -- Mycoplasma detection / J.M. Mowles -- Short-term chorionic villi and amniotie fluid cultures / M.L. Murer-Orlando, V.M. McGuire -- Keratinocyte culture / Yvonne Barlow, Richard J. Pye -- Establishment of mouse epithelial lung cell strains and cell lines / Garry J. Smith -- Culture of human brain tumors on an extracellular matrix derived from bovine corneal endothelial cells and cultured human glioma cells / Manfred Westphal, Marianne Hänsel, Hildegard Nausch, Eva Rohde, Hans-Dietrich Herrmann -- Embryonic rodent brain cells in culture / Narayan R. Bhat -- Human thyroid epithelial cells / D.W. Williams, David Wynford-Thomas -- Preparation of isolated rat liver hepatocytes / Bjørn Quistorff, John Dich, Niels Grunnet -- Primary cultures of rat hepatocytes / John Dich, Niels Grunnet -- Preparation of isolated periportal or perivenous hepatocytes from rat liver / Bjørn Quistorff -- Primary kidney cells / Mary Taub -- Adipocytes / Shinobu Gamou, Yoshiko Shimizu, Nobuyoshi Shimizu -- Tissue culture of skeletal muscle / Terry A. Partridge -- Derivation and maintenance of embryonic stem cell cultures / Elizabeth J. Robertson -- Defining hormone and matrix requirements for differentiated epithelia / Lola M. Reid -- Culturing primitive hemopoietic cells: long-term mouse marrow cultures and the establishment of factor-dependent (FDCP-mix) hemopoietic cell lines / Elaine Spooncer, T. Michael Dexter -- High proliferative potential colony forming cells / T. Ray Bradley, George S. Hodgson, Ivan Bertoncello -- Murine bone marrow-derived macrophages / E. Richard Stanley -- Long-term B lymphoid cultures from murine bone marrow: establishment and cloning by using stromal cell line AC 6.21 / Cheryl A. Whitlock, Christa E. Muller-Sieburg -- CFU-C in agar / Vincent Praloran, Anna Bartocci -- Human long-term bone marrow culture / Armand Keating, Paul Toor -- In vitro clonal culture of human hematopoietic progenitor cells / Armand Keating, Paul Toor -- Flow sorting for isolating CFU-E / Suzanne M. Watt, John M. Davis -- Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors / Malcolm Strath, Elaine J. Clutterbuck, Colin J. Sanderson -- Clonogenic assays for hematopoietic and tumor cells using agar-containing capillaries / H. Rainer Maurer, Christine Echarti -- Cytogenetic techniques for human leukemias / G. John Swansbury -- Time-lapse cinemicroscopy / Peter N. Riddle -- Transmission and scanning electron microscope preparation of whole cultured cells / Josef Neumülller -- Double indirect-immunofluorescent labeling of cultured cells / Christine A. Boocock -- Gene mapping to chromosomes by hybridization in situ / John R. Gosden -- In situ hybridization with radiolabeled cRNA probes, using tissue sections and smears / Carolin Adams, Qutayla A. Hamid, Julia M. Polak -- Immunogold labeling for electron microscopy / John Pacy -- Human chromosome analysis and sorting / Judith A. Fantes, Daryll K. Green -- Flow cytometry / Michael G. Ormerod, Patrick R. Imrie -- Fluorescence-activated cell sorting according to receptor density : application for isolating transfected cell lines / Stella Clark -- Transfection and transformation of human thyroid epithelial cells / Nicholas Robert Lemoine, David Wynford-Thomas -- Expression of foreign genes in cultured insect cells using a recombinant baculovirus vector / Martin J. Page, Vivienne F. Murphy -- Chloramphenicol acetyltransferase as a reporter in mammalian gene transfer / Christopher D. Corsico, Bruce H. Howard -- Immunizing schedules for hybridoma production / Björn Gustafsson -- Fusion protocol for the production of mouse hybridomas / Björn Gustafsson -- Cloning of hybridomas / Björn Gustafsson -- Enzyme-linked immunosorbent assay for screening of antibodies in hybridoma supernatants / Björn Gustafsson -- Cryopreservation of hybridomas / Björn Gustafsson -- Isotype determination of monoclonal antibodies by immunodiffusion / Björn Gustafsson -- Isoelectric focusing and immunofixation of monoclonal antibodies / Björn Gustafsson -- Large-scale production and storage of monoclonal antibodies and hybridomas / Stuart A. Clark, J. Bryan Griffiths, Christopher B. Morris -- Purification of murine monoclonal antibodies / Michael G. Baines, Andrew J. Gearing, Robin Thorpe -- Rat [cross] rat hybridomas / Christopher J. Dean -- Monoclonal antibodies against glycosphingolipids (GSLs)-gangliosides / Bruce Fletcher Clark.Animal Cell Culture [texte imprimé] . - [S.l. : s.n.], 2013 . - xiv, 713 p. : ill.. - () .
ISBN : 978-1-59259-492-4
Langues : Anglais
Tags : Cell culture -- Tissue culture -- Technique Index. décimale : Résumé : For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques for establishing such a facility. Much had to be done by trial and error or gleaned from originalpapers. Consequently, I felt that a methods book covering a wide variety of techniques from basic culture to the most sophisticated cell analysis would be a very valuable addition to the scientific literature. In the interim, several useful books (listed in Chapter I of this volume) did appear, but none entirely fitted the bill and some are now somewhat dated. Then, in 1984, the first of the Methods in Molecular Biology volumes from Humana Press was published with its step-by-step recipe approach. This format appealed to me, and so I c- tacted John Walker, the series editor, about including cell culture in this series. The result was that we embarked upon a single volume covering both plant and animal cell culture. Such was the richness of the material that this project soon divided itself into separate volumes on animal cell (Volume 5) and plant cell (Volume 6) culture. In this volume (Volume 5), therefore, we have aimed to describe a variety of basic techniques and culture conditions for a range of cell types.
Contents
Basic cell culture / Jeffrey W. Pollard -- Establishment, maintenance, and cloning of human primary cell strains / Gareth E. Jones -- Aging of cultured human skin fibroblasts / Calvin B. Harley -- Separation and maintenance of primary T and B lymphocytes / Derek Kinchington, Eleanor Berrie -- Establishment of lymphoblastoid cell lines / A. Doyle -- Scale-up of suspension and anchorage-dependent animal cells / J. Bryan Griffiths -- Mycoplasma detection / J.M. Mowles -- Short-term chorionic villi and amniotie fluid cultures / M.L. Murer-Orlando, V.M. McGuire -- Keratinocyte culture / Yvonne Barlow, Richard J. Pye -- Establishment of mouse epithelial lung cell strains and cell lines / Garry J. Smith -- Culture of human brain tumors on an extracellular matrix derived from bovine corneal endothelial cells and cultured human glioma cells / Manfred Westphal, Marianne Hänsel, Hildegard Nausch, Eva Rohde, Hans-Dietrich Herrmann -- Embryonic rodent brain cells in culture / Narayan R. Bhat -- Human thyroid epithelial cells / D.W. Williams, David Wynford-Thomas -- Preparation of isolated rat liver hepatocytes / Bjørn Quistorff, John Dich, Niels Grunnet -- Primary cultures of rat hepatocytes / John Dich, Niels Grunnet -- Preparation of isolated periportal or perivenous hepatocytes from rat liver / Bjørn Quistorff -- Primary kidney cells / Mary Taub -- Adipocytes / Shinobu Gamou, Yoshiko Shimizu, Nobuyoshi Shimizu -- Tissue culture of skeletal muscle / Terry A. Partridge -- Derivation and maintenance of embryonic stem cell cultures / Elizabeth J. Robertson -- Defining hormone and matrix requirements for differentiated epithelia / Lola M. Reid -- Culturing primitive hemopoietic cells: long-term mouse marrow cultures and the establishment of factor-dependent (FDCP-mix) hemopoietic cell lines / Elaine Spooncer, T. Michael Dexter -- High proliferative potential colony forming cells / T. Ray Bradley, George S. Hodgson, Ivan Bertoncello -- Murine bone marrow-derived macrophages / E. Richard Stanley -- Long-term B lymphoid cultures from murine bone marrow: establishment and cloning by using stromal cell line AC 6.21 / Cheryl A. Whitlock, Christa E. Muller-Sieburg -- CFU-C in agar / Vincent Praloran, Anna Bartocci -- Human long-term bone marrow culture / Armand Keating, Paul Toor -- In vitro clonal culture of human hematopoietic progenitor cells / Armand Keating, Paul Toor -- Flow sorting for isolating CFU-E / Suzanne M. Watt, John M. Davis -- Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors / Malcolm Strath, Elaine J. Clutterbuck, Colin J. Sanderson -- Clonogenic assays for hematopoietic and tumor cells using agar-containing capillaries / H. Rainer Maurer, Christine Echarti -- Cytogenetic techniques for human leukemias / G. John Swansbury -- Time-lapse cinemicroscopy / Peter N. Riddle -- Transmission and scanning electron microscope preparation of whole cultured cells / Josef Neumülller -- Double indirect-immunofluorescent labeling of cultured cells / Christine A. Boocock -- Gene mapping to chromosomes by hybridization in situ / John R. Gosden -- In situ hybridization with radiolabeled cRNA probes, using tissue sections and smears / Carolin Adams, Qutayla A. Hamid, Julia M. Polak -- Immunogold labeling for electron microscopy / John Pacy -- Human chromosome analysis and sorting / Judith A. Fantes, Daryll K. Green -- Flow cytometry / Michael G. Ormerod, Patrick R. Imrie -- Fluorescence-activated cell sorting according to receptor density : application for isolating transfected cell lines / Stella Clark -- Transfection and transformation of human thyroid epithelial cells / Nicholas Robert Lemoine, David Wynford-Thomas -- Expression of foreign genes in cultured insect cells using a recombinant baculovirus vector / Martin J. Page, Vivienne F. Murphy -- Chloramphenicol acetyltransferase as a reporter in mammalian gene transfer / Christopher D. Corsico, Bruce H. Howard -- Immunizing schedules for hybridoma production / Björn Gustafsson -- Fusion protocol for the production of mouse hybridomas / Björn Gustafsson -- Cloning of hybridomas / Björn Gustafsson -- Enzyme-linked immunosorbent assay for screening of antibodies in hybridoma supernatants / Björn Gustafsson -- Cryopreservation of hybridomas / Björn Gustafsson -- Isotype determination of monoclonal antibodies by immunodiffusion / Björn Gustafsson -- Isoelectric focusing and immunofixation of monoclonal antibodies / Björn Gustafsson -- Large-scale production and storage of monoclonal antibodies and hybridomas / Stuart A. Clark, J. Bryan Griffiths, Christopher B. Morris -- Purification of murine monoclonal antibodies / Michael G. Baines, Andrew J. Gearing, Robin Thorpe -- Rat [cross] rat hybridomas / Christopher J. Dean -- Monoclonal antibodies against glycosphingolipids (GSLs)-gangliosides / Bruce Fletcher Clark.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Plant Cell and Tissue Culture
Titre : Plant Cell and Tissue Culture Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : 1990 Collection : Importance : XII, 597 p. ISBN/ISSN/EAN : 978-1-4899-4387-3 Langues : Anglais Tags : Science - Biology - Botany & Plant Sciences - Cellular Biology - Molecular Biology Index. décimale : Résumé : Plant Cell and Tissue Culture continues the high standards of Humana's Methods in Molecular Biology series. Its step-by-step approach (a hallmark of the series) is applied to a wide range of basic laboratory techniques and culture conditions appropriate to plant cells.
Because of the diversity of cell types, species, and culture methods, much of this volume is devoted to the culture of particular cell types and to the regeneration of these cells into whole plants. Special attention is also given to the genetic modification of plants, as well as to the economic significance of plant products.
Chapters cover a wide range of topics and techniques, including:* tissue culture media and selection * cryopreservation * callus culture techniques * organ culture * embryogenesis * batch culture * large-scale culture * hormonal control * fertilization techniques * gene transfer * cell immobilization * production systems * cell product purification * DNA expression * electrofusion of plant cells * mutant selection * mutagenesis techniques * automation * transfer of nuclei * protoplast culture * media analysis * micropropagation.
A detailed appendix lists the formulas for the most commonly employed plant cell media. Comprehensive, easy to follow, and a pleasure to use, Pollard and Walker's Plant Cell and Tissue Culture is an essential tool for everyone--at all levels of proficiency and experience--involved in plant culture.
Table of Contents
Selection of Media for Tissue and Cell Culture. Growth Determination and Medium Analysis. Measurements of Viability Suitable for Plant Tissue Cultures. Cryopreservation of Plant Cells. Hormonal Control of Growth and Development. The Initiation and Maintenance of Callus Cultures. Organogenesis in Callus Culture. Manipulation of Shoot Formation in Cultured Explants. Meristem Tip Culture. Micropropagation: Axillary Bud Multiplication. Grafting In Vitro. Flower Organ Culture. Fruit Organ Cultures. Culture of Zygotic Embryos of Higher Plants. Induction of Embryogenesis in Callus Culture. Induction of Embryogenesis in Suspension Culture. Microspore Culture in Brassica. Growth of Ferns from Spores in Axenic Culture. Clonal Propagation of Orchids. Tissue Culture and Top-Fruit Tree Species. Fertilization In Vitro and Culture of Fertilized Ovules of Higher Plants. Vegetative Propagation of Cacti and Other Succulents In Vitro. The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization. Protoplasts of Higher and Lower Plants: Isolation, Culture, and Fusion. Plant Protoplast Enucleation by Density Gradient Centrifugation. Isolation and Transplantation of Nuclei into Plant Protoplasts. Induction of Hairy Roots by Agrobacterium Rhizogenes and Growth of Hairy Roots In Vitro. Agrobacterium Rhizogenes-Mediated Gene Transfer Using PRI 1855 and a Binary Vector. Transformation of Rape with Agrobacterium tumefaciens-Based Vectors. Transient and Stable Expression of Foreign DNA Introduced into Plant Protoplasts by Electroporation. Protoplast Microinjection Using Agarose Microdrops. Transformation of Plants Via the Shoot Apex. Methods of Gene Transfer and Analysis in Higher Plants. Electrofusion of Plant Cells. Production of Cybrids in Rapeseed (Brassica rapus). Selection of Somatic Hybrids by Resistance Complementation. Dual Fungal and Plant Cell Culture. Mutagenesis Techniques in Plant Tissue Cultures. Mutagenesis: EMS Treatment of Cell Suspensions of Nicotiana Sylvestris. Techniques for Selecting Mutants from Plant Tissue Cultures. Selection of Antimetabolite Resistant Mutants. Selection of Chloroplast Mutants. Large-Scale Culture of Plant Cells. Batch Suspension Culture. Production Systems. Immobilization of Cells by Spontaneous Adhesion. Plant Cell Immobilization in Alginate and Polyurethane Foam. Alkaloid Secondary Products from Catharanthus roseus Cell Suspension. Betanin Production and Release In Vitro from Suspension Cultures of Beta vulgaris. Enzyme Extraction and Assessment of Enzyme Production. Micromachines to Automate Plant Tissue Culture. Automation of the Surface Sterilization System Procedure. Appendix. Index.Plant Cell and Tissue Culture [texte imprimé] . - [S.l. : s.n.], 1990 . - XII, 597 p.. - () .
ISBN : 978-1-4899-4387-3
Langues : Anglais
Tags : Science - Biology - Botany & Plant Sciences - Cellular Biology - Molecular Biology Index. décimale : Résumé : Plant Cell and Tissue Culture continues the high standards of Humana's Methods in Molecular Biology series. Its step-by-step approach (a hallmark of the series) is applied to a wide range of basic laboratory techniques and culture conditions appropriate to plant cells.
Because of the diversity of cell types, species, and culture methods, much of this volume is devoted to the culture of particular cell types and to the regeneration of these cells into whole plants. Special attention is also given to the genetic modification of plants, as well as to the economic significance of plant products.
Chapters cover a wide range of topics and techniques, including:* tissue culture media and selection * cryopreservation * callus culture techniques * organ culture * embryogenesis * batch culture * large-scale culture * hormonal control * fertilization techniques * gene transfer * cell immobilization * production systems * cell product purification * DNA expression * electrofusion of plant cells * mutant selection * mutagenesis techniques * automation * transfer of nuclei * protoplast culture * media analysis * micropropagation.
A detailed appendix lists the formulas for the most commonly employed plant cell media. Comprehensive, easy to follow, and a pleasure to use, Pollard and Walker's Plant Cell and Tissue Culture is an essential tool for everyone--at all levels of proficiency and experience--involved in plant culture.
Table of Contents
Selection of Media for Tissue and Cell Culture. Growth Determination and Medium Analysis. Measurements of Viability Suitable for Plant Tissue Cultures. Cryopreservation of Plant Cells. Hormonal Control of Growth and Development. The Initiation and Maintenance of Callus Cultures. Organogenesis in Callus Culture. Manipulation of Shoot Formation in Cultured Explants. Meristem Tip Culture. Micropropagation: Axillary Bud Multiplication. Grafting In Vitro. Flower Organ Culture. Fruit Organ Cultures. Culture of Zygotic Embryos of Higher Plants. Induction of Embryogenesis in Callus Culture. Induction of Embryogenesis in Suspension Culture. Microspore Culture in Brassica. Growth of Ferns from Spores in Axenic Culture. Clonal Propagation of Orchids. Tissue Culture and Top-Fruit Tree Species. Fertilization In Vitro and Culture of Fertilized Ovules of Higher Plants. Vegetative Propagation of Cacti and Other Succulents In Vitro. The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization. Protoplasts of Higher and Lower Plants: Isolation, Culture, and Fusion. Plant Protoplast Enucleation by Density Gradient Centrifugation. Isolation and Transplantation of Nuclei into Plant Protoplasts. Induction of Hairy Roots by Agrobacterium Rhizogenes and Growth of Hairy Roots In Vitro. Agrobacterium Rhizogenes-Mediated Gene Transfer Using PRI 1855 and a Binary Vector. Transformation of Rape with Agrobacterium tumefaciens-Based Vectors. Transient and Stable Expression of Foreign DNA Introduced into Plant Protoplasts by Electroporation. Protoplast Microinjection Using Agarose Microdrops. Transformation of Plants Via the Shoot Apex. Methods of Gene Transfer and Analysis in Higher Plants. Electrofusion of Plant Cells. Production of Cybrids in Rapeseed (Brassica rapus). Selection of Somatic Hybrids by Resistance Complementation. Dual Fungal and Plant Cell Culture. Mutagenesis Techniques in Plant Tissue Cultures. Mutagenesis: EMS Treatment of Cell Suspensions of Nicotiana Sylvestris. Techniques for Selecting Mutants from Plant Tissue Cultures. Selection of Antimetabolite Resistant Mutants. Selection of Chloroplast Mutants. Large-Scale Culture of Plant Cells. Batch Suspension Culture. Production Systems. Immobilization of Cells by Spontaneous Adhesion. Plant Cell Immobilization in Alginate and Polyurethane Foam. Alkaloid Secondary Products from Catharanthus roseus Cell Suspension. Betanin Production and Release In Vitro from Suspension Cultures of Beta vulgaris. Enzyme Extraction and Assessment of Enzyme Production. Micromachines to Automate Plant Tissue Culture. Automation of the Surface Sterilization System Procedure. Appendix. Index.Exemplaires
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