Bibliothèque de l'ENSBS
http://ensbiotech.edu.dz
A partir de cette page vous pouvez :
| Retourner au premier écran avec les catégories... |
Détail d'une collection
Documents disponibles dans la collection
Affiner la recherche Interroger des sources externesAdvanced protocols in oxidative stress III
Titre : Advanced protocols in oxidative stress III Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : cop. 2015 Autre Editeur : Springer Collection : num. 1208 Importance : 1 vol. (xiv-477 p.) Présentation : ill Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1440-1 Langues : Anglais Tags : Stress oxydatif physiologie Antioxydants Espèces réactives de l'azote métabolisme Expression des gènes Biostatistiques Index. décimale : Résumé : Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.Advanced protocols in oxidative stress III [texte imprimé] . - [S.l. : s.n.] : [S.l.] : Springer, cop. 2015 . - 1 vol. (xiv-477 p.) : ill ; 26 cm. - (; 1208) .
ISBN : 978-1-4939-1440-1
Langues : Anglais
Tags : Stress oxydatif physiologie Antioxydants Espèces réactives de l'azote métabolisme Expression des gènes Biostatistiques Index. décimale : Résumé : Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Titre : Animal Cell Culture Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : 2013 Collection : Importance : xiv, 713 p. Présentation : ill. ISBN/ISSN/EAN : 978-1-59259-492-4 Langues : Anglais Tags : Cell culture -- Tissue culture -- Technique Index. décimale : Résumé : For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques for establishing such a facility. Much had to be done by trial and error or gleaned from originalpapers. Consequently, I felt that a methods book covering a wide variety of techniques from basic culture to the most sophisticated cell analysis would be a very valuable addition to the scientific literature. In the interim, several useful books (listed in Chapter I of this volume) did appear, but none entirely fitted the bill and some are now somewhat dated. Then, in 1984, the first of the Methods in Molecular Biology volumes from Humana Press was published with its step-by-step recipe approach. This format appealed to me, and so I c- tacted John Walker, the series editor, about including cell culture in this series. The result was that we embarked upon a single volume covering both plant and animal cell culture. Such was the richness of the material that this project soon divided itself into separate volumes on animal cell (Volume 5) and plant cell (Volume 6) culture. In this volume (Volume 5), therefore, we have aimed to describe a variety of basic techniques and culture conditions for a range of cell types.
Contents
Basic cell culture / Jeffrey W. Pollard -- Establishment, maintenance, and cloning of human primary cell strains / Gareth E. Jones -- Aging of cultured human skin fibroblasts / Calvin B. Harley -- Separation and maintenance of primary T and B lymphocytes / Derek Kinchington, Eleanor Berrie -- Establishment of lymphoblastoid cell lines / A. Doyle -- Scale-up of suspension and anchorage-dependent animal cells / J. Bryan Griffiths -- Mycoplasma detection / J.M. Mowles -- Short-term chorionic villi and amniotie fluid cultures / M.L. Murer-Orlando, V.M. McGuire -- Keratinocyte culture / Yvonne Barlow, Richard J. Pye -- Establishment of mouse epithelial lung cell strains and cell lines / Garry J. Smith -- Culture of human brain tumors on an extracellular matrix derived from bovine corneal endothelial cells and cultured human glioma cells / Manfred Westphal, Marianne Hänsel, Hildegard Nausch, Eva Rohde, Hans-Dietrich Herrmann -- Embryonic rodent brain cells in culture / Narayan R. Bhat -- Human thyroid epithelial cells / D.W. Williams, David Wynford-Thomas -- Preparation of isolated rat liver hepatocytes / Bjørn Quistorff, John Dich, Niels Grunnet -- Primary cultures of rat hepatocytes / John Dich, Niels Grunnet -- Preparation of isolated periportal or perivenous hepatocytes from rat liver / Bjørn Quistorff -- Primary kidney cells / Mary Taub -- Adipocytes / Shinobu Gamou, Yoshiko Shimizu, Nobuyoshi Shimizu -- Tissue culture of skeletal muscle / Terry A. Partridge -- Derivation and maintenance of embryonic stem cell cultures / Elizabeth J. Robertson -- Defining hormone and matrix requirements for differentiated epithelia / Lola M. Reid -- Culturing primitive hemopoietic cells: long-term mouse marrow cultures and the establishment of factor-dependent (FDCP-mix) hemopoietic cell lines / Elaine Spooncer, T. Michael Dexter -- High proliferative potential colony forming cells / T. Ray Bradley, George S. Hodgson, Ivan Bertoncello -- Murine bone marrow-derived macrophages / E. Richard Stanley -- Long-term B lymphoid cultures from murine bone marrow: establishment and cloning by using stromal cell line AC 6.21 / Cheryl A. Whitlock, Christa E. Muller-Sieburg -- CFU-C in agar / Vincent Praloran, Anna Bartocci -- Human long-term bone marrow culture / Armand Keating, Paul Toor -- In vitro clonal culture of human hematopoietic progenitor cells / Armand Keating, Paul Toor -- Flow sorting for isolating CFU-E / Suzanne M. Watt, John M. Davis -- Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors / Malcolm Strath, Elaine J. Clutterbuck, Colin J. Sanderson -- Clonogenic assays for hematopoietic and tumor cells using agar-containing capillaries / H. Rainer Maurer, Christine Echarti -- Cytogenetic techniques for human leukemias / G. John Swansbury -- Time-lapse cinemicroscopy / Peter N. Riddle -- Transmission and scanning electron microscope preparation of whole cultured cells / Josef Neumülller -- Double indirect-immunofluorescent labeling of cultured cells / Christine A. Boocock -- Gene mapping to chromosomes by hybridization in situ / John R. Gosden -- In situ hybridization with radiolabeled cRNA probes, using tissue sections and smears / Carolin Adams, Qutayla A. Hamid, Julia M. Polak -- Immunogold labeling for electron microscopy / John Pacy -- Human chromosome analysis and sorting / Judith A. Fantes, Daryll K. Green -- Flow cytometry / Michael G. Ormerod, Patrick R. Imrie -- Fluorescence-activated cell sorting according to receptor density : application for isolating transfected cell lines / Stella Clark -- Transfection and transformation of human thyroid epithelial cells / Nicholas Robert Lemoine, David Wynford-Thomas -- Expression of foreign genes in cultured insect cells using a recombinant baculovirus vector / Martin J. Page, Vivienne F. Murphy -- Chloramphenicol acetyltransferase as a reporter in mammalian gene transfer / Christopher D. Corsico, Bruce H. Howard -- Immunizing schedules for hybridoma production / Björn Gustafsson -- Fusion protocol for the production of mouse hybridomas / Björn Gustafsson -- Cloning of hybridomas / Björn Gustafsson -- Enzyme-linked immunosorbent assay for screening of antibodies in hybridoma supernatants / Björn Gustafsson -- Cryopreservation of hybridomas / Björn Gustafsson -- Isotype determination of monoclonal antibodies by immunodiffusion / Björn Gustafsson -- Isoelectric focusing and immunofixation of monoclonal antibodies / Björn Gustafsson -- Large-scale production and storage of monoclonal antibodies and hybridomas / Stuart A. Clark, J. Bryan Griffiths, Christopher B. Morris -- Purification of murine monoclonal antibodies / Michael G. Baines, Andrew J. Gearing, Robin Thorpe -- Rat [cross] rat hybridomas / Christopher J. Dean -- Monoclonal antibodies against glycosphingolipids (GSLs)-gangliosides / Bruce Fletcher Clark.Animal Cell Culture [texte imprimé] . - [S.l. : s.n.], 2013 . - xiv, 713 p. : ill.. - () .
ISBN : 978-1-59259-492-4
Langues : Anglais
Tags : Cell culture -- Tissue culture -- Technique Index. décimale : Résumé : For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques for establishing such a facility. Much had to be done by trial and error or gleaned from originalpapers. Consequently, I felt that a methods book covering a wide variety of techniques from basic culture to the most sophisticated cell analysis would be a very valuable addition to the scientific literature. In the interim, several useful books (listed in Chapter I of this volume) did appear, but none entirely fitted the bill and some are now somewhat dated. Then, in 1984, the first of the Methods in Molecular Biology volumes from Humana Press was published with its step-by-step recipe approach. This format appealed to me, and so I c- tacted John Walker, the series editor, about including cell culture in this series. The result was that we embarked upon a single volume covering both plant and animal cell culture. Such was the richness of the material that this project soon divided itself into separate volumes on animal cell (Volume 5) and plant cell (Volume 6) culture. In this volume (Volume 5), therefore, we have aimed to describe a variety of basic techniques and culture conditions for a range of cell types.
Contents
Basic cell culture / Jeffrey W. Pollard -- Establishment, maintenance, and cloning of human primary cell strains / Gareth E. Jones -- Aging of cultured human skin fibroblasts / Calvin B. Harley -- Separation and maintenance of primary T and B lymphocytes / Derek Kinchington, Eleanor Berrie -- Establishment of lymphoblastoid cell lines / A. Doyle -- Scale-up of suspension and anchorage-dependent animal cells / J. Bryan Griffiths -- Mycoplasma detection / J.M. Mowles -- Short-term chorionic villi and amniotie fluid cultures / M.L. Murer-Orlando, V.M. McGuire -- Keratinocyte culture / Yvonne Barlow, Richard J. Pye -- Establishment of mouse epithelial lung cell strains and cell lines / Garry J. Smith -- Culture of human brain tumors on an extracellular matrix derived from bovine corneal endothelial cells and cultured human glioma cells / Manfred Westphal, Marianne Hänsel, Hildegard Nausch, Eva Rohde, Hans-Dietrich Herrmann -- Embryonic rodent brain cells in culture / Narayan R. Bhat -- Human thyroid epithelial cells / D.W. Williams, David Wynford-Thomas -- Preparation of isolated rat liver hepatocytes / Bjørn Quistorff, John Dich, Niels Grunnet -- Primary cultures of rat hepatocytes / John Dich, Niels Grunnet -- Preparation of isolated periportal or perivenous hepatocytes from rat liver / Bjørn Quistorff -- Primary kidney cells / Mary Taub -- Adipocytes / Shinobu Gamou, Yoshiko Shimizu, Nobuyoshi Shimizu -- Tissue culture of skeletal muscle / Terry A. Partridge -- Derivation and maintenance of embryonic stem cell cultures / Elizabeth J. Robertson -- Defining hormone and matrix requirements for differentiated epithelia / Lola M. Reid -- Culturing primitive hemopoietic cells: long-term mouse marrow cultures and the establishment of factor-dependent (FDCP-mix) hemopoietic cell lines / Elaine Spooncer, T. Michael Dexter -- High proliferative potential colony forming cells / T. Ray Bradley, George S. Hodgson, Ivan Bertoncello -- Murine bone marrow-derived macrophages / E. Richard Stanley -- Long-term B lymphoid cultures from murine bone marrow: establishment and cloning by using stromal cell line AC 6.21 / Cheryl A. Whitlock, Christa E. Muller-Sieburg -- CFU-C in agar / Vincent Praloran, Anna Bartocci -- Human long-term bone marrow culture / Armand Keating, Paul Toor -- In vitro clonal culture of human hematopoietic progenitor cells / Armand Keating, Paul Toor -- Flow sorting for isolating CFU-E / Suzanne M. Watt, John M. Davis -- Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors / Malcolm Strath, Elaine J. Clutterbuck, Colin J. Sanderson -- Clonogenic assays for hematopoietic and tumor cells using agar-containing capillaries / H. Rainer Maurer, Christine Echarti -- Cytogenetic techniques for human leukemias / G. John Swansbury -- Time-lapse cinemicroscopy / Peter N. Riddle -- Transmission and scanning electron microscope preparation of whole cultured cells / Josef Neumülller -- Double indirect-immunofluorescent labeling of cultured cells / Christine A. Boocock -- Gene mapping to chromosomes by hybridization in situ / John R. Gosden -- In situ hybridization with radiolabeled cRNA probes, using tissue sections and smears / Carolin Adams, Qutayla A. Hamid, Julia M. Polak -- Immunogold labeling for electron microscopy / John Pacy -- Human chromosome analysis and sorting / Judith A. Fantes, Daryll K. Green -- Flow cytometry / Michael G. Ormerod, Patrick R. Imrie -- Fluorescence-activated cell sorting according to receptor density : application for isolating transfected cell lines / Stella Clark -- Transfection and transformation of human thyroid epithelial cells / Nicholas Robert Lemoine, David Wynford-Thomas -- Expression of foreign genes in cultured insect cells using a recombinant baculovirus vector / Martin J. Page, Vivienne F. Murphy -- Chloramphenicol acetyltransferase as a reporter in mammalian gene transfer / Christopher D. Corsico, Bruce H. Howard -- Immunizing schedules for hybridoma production / Björn Gustafsson -- Fusion protocol for the production of mouse hybridomas / Björn Gustafsson -- Cloning of hybridomas / Björn Gustafsson -- Enzyme-linked immunosorbent assay for screening of antibodies in hybridoma supernatants / Björn Gustafsson -- Cryopreservation of hybridomas / Björn Gustafsson -- Isotype determination of monoclonal antibodies by immunodiffusion / Björn Gustafsson -- Isoelectric focusing and immunofixation of monoclonal antibodies / Björn Gustafsson -- Large-scale production and storage of monoclonal antibodies and hybridomas / Stuart A. Clark, J. Bryan Griffiths, Christopher B. Morris -- Purification of murine monoclonal antibodies / Michael G. Baines, Andrew J. Gearing, Robin Thorpe -- Rat [cross] rat hybridomas / Christopher J. Dean -- Monoclonal antibodies against glycosphingolipids (GSLs)-gangliosides / Bruce Fletcher Clark.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Titre : Bacterial pangenomics : methods and protocols Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : cop. 2015 Collection : num. 1231 Importance : 1 vol. (x-298 p.) Présentation : ill. en noir et en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1719-8 Langues : Anglais Tags : Génome bactérien Électrophorèse Voies et réseaux métaboliques Techniques de laboratoire clinique Index. décimale : Résumé : Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.Bacterial pangenomics : methods and protocols [texte imprimé] . - [S.l. : s.n.], cop. 2015 . - 1 vol. (x-298 p.) : ill. en noir et en coul ; 26 cm. - (; 1231) .
ISBN : 978-1-4939-1719-8
Langues : Anglais
Tags : Génome bactérien Électrophorèse Voies et réseaux métaboliques Techniques de laboratoire clinique Index. décimale : Résumé : Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Titre : DNA Sequencing Protocols Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : 2001 Collection : Importance : X, 244 p. Présentation : 33 Illustrations, black and white ISBN/ISSN/EAN : 978-1-4899-4212-8 Langues : Anglais Tags : DNA - Sequencing Protocols Index. décimale : Résumé : Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects. DNA Sequencing Protocols [texte imprimé] . - [S.l. : s.n.], 2001 . - X, 244 p. : 33 Illustrations, black and white. - () .
ISBN : 978-1-4899-4212-8
Langues : Anglais
Tags : DNA - Sequencing Protocols Index. décimale : Résumé : Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects. Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Titre : Environmental microbiology : methods and protocols Type de document : texte imprimé Mention d'édition : Second edition Editeur : New York : Humana Press Année de publication : cop. 2014 Autre Editeur : Heidelberg : Springer Collection : num. 1096 Importance : 1 vol. (X-242 p.) Présentation : ill. en noir et en coul., graphs., schémas, tabs., couv. ill. en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-62703-712-9 Langues : Anglais Tags : Life Sciences Microbiology Environmental Science and Engineering Microbiologie de l'environnement Manuel de laboratoire Bactéries isolement et purification Techniques microbiologiques Environnement Microbiologie Life sciences Index. décimale : Résumé : An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more.Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities.This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes Environmental microbiology : methods and protocols [texte imprimé] . - Second edition . - [S.l. : s.n.] : Heidelberg : Springer, cop. 2014 . - 1 vol. (X-242 p.) : ill. en noir et en coul., graphs., schémas, tabs., couv. ill. en coul ; 26 cm. - (; 1096) .
ISBN : 978-1-62703-712-9
Langues : Anglais
Tags : Life Sciences Microbiology Environmental Science and Engineering Microbiologie de l'environnement Manuel de laboratoire Bactéries isolement et purification Techniques microbiologiques Environnement Microbiologie Life sciences Index. décimale : Résumé : An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more.Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities.This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
