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Cycle cellulaire et cytométrie en flux
Titre : Cycle cellulaire et cytométrie en flux Type de document : texte imprimé Editeur : Paris : Éd. Tec & doc Année de publication : DL 2010 Importance : 1 vol. (XIV-282 p.) Présentation : ill., couv. ill. en coul. Format : 24 cm ISBN/ISSN/EAN : 978-2-7430-1205-2 Note générale : Notes bibliogr. Index Langues : Français Tags : Cytométrie de flux Cycle cellulaire Index. décimale : Résumé : Le cycle cellulaire est sans conteste le processus primordial sur lequel repose toute la biologie, son ultime étape étant la division, fondement de la multiplication cellulaire.À travers elle, les organismes vivants se développent dans toute leur complexité, car, à chaque cycle, au-delà de leur accumulation, les cellules s'orientent progressivement vers des fonctions spécifiques ou la mort pour certaines d'entre elles. Cette délicate balance entre prolifération, différenciation et mort est depuis longtemps l'objet de l'attention des biologistes, car la rupture de cet équilibre peut déboucher sur un phénomène de prolifération incontrôlée, à l'origine de pathologies tumorales.Parmi les nombreuses approches utilisées pour étudier les mécanismes complexes impliqués dans les contrôles du cycle cellulaire, la cytométrie en flux (CMF) a permis de faire avancer la connaissance de manière particulièrement significative. Les potentialités de cette technique, autant par l'aspect multiparamétrique des analyses que par la possibilité de tri qu'elle offre, ont permis d'aborder le cycle cellulaire sous de multiples aspects : estimation précise des phases du cycle, mesure de la prolifération, expression relative des protéines régulatrices, mesure de ploïdie - études réalisées aussi bien sur des cellules eucaryotes, animales ou végétales, que sur des procaryotes tels que les bactéries.Cycle cellulaire et cytométrie en flux a été conçu comme un guide pratique qui offre une vision aussi large que possible des champs d'application de la CMF à l'étude du cycle, aussi bien à partir de concepts théoriques que d'exemples pratiques. Écrits par des spécialistes du domaine, les différents chapitres mettent en avant les avantages, mais aussi les problèmes et les pièges, que l'utilisateur pourrait rencontrer. De plus, des protocoles essentiels Cycle cellulaire et cytométrie en flux [texte imprimé] . - Paris : Éd. Tec & doc, DL 2010 . - 1 vol. (XIV-282 p.) : ill., couv. ill. en coul. ; 24 cm.
ISBN : 978-2-7430-1205-2
Notes bibliogr. Index
Langues : Français
Tags : Cytométrie de flux Cycle cellulaire Index. décimale : Résumé : Le cycle cellulaire est sans conteste le processus primordial sur lequel repose toute la biologie, son ultime étape étant la division, fondement de la multiplication cellulaire.À travers elle, les organismes vivants se développent dans toute leur complexité, car, à chaque cycle, au-delà de leur accumulation, les cellules s'orientent progressivement vers des fonctions spécifiques ou la mort pour certaines d'entre elles. Cette délicate balance entre prolifération, différenciation et mort est depuis longtemps l'objet de l'attention des biologistes, car la rupture de cet équilibre peut déboucher sur un phénomène de prolifération incontrôlée, à l'origine de pathologies tumorales.Parmi les nombreuses approches utilisées pour étudier les mécanismes complexes impliqués dans les contrôles du cycle cellulaire, la cytométrie en flux (CMF) a permis de faire avancer la connaissance de manière particulièrement significative. Les potentialités de cette technique, autant par l'aspect multiparamétrique des analyses que par la possibilité de tri qu'elle offre, ont permis d'aborder le cycle cellulaire sous de multiples aspects : estimation précise des phases du cycle, mesure de la prolifération, expression relative des protéines régulatrices, mesure de ploïdie - études réalisées aussi bien sur des cellules eucaryotes, animales ou végétales, que sur des procaryotes tels que les bactéries.Cycle cellulaire et cytométrie en flux a été conçu comme un guide pratique qui offre une vision aussi large que possible des champs d'application de la CMF à l'étude du cycle, aussi bien à partir de concepts théoriques que d'exemples pratiques. Écrits par des spécialistes du domaine, les différents chapitres mettent en avant les avantages, mais aussi les problèmes et les pièges, que l'utilisateur pourrait rencontrer. De plus, des protocoles essentiels Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Advances in applications, Part B. Recent advances in cytometry
Titre de série : Advances in applications, Part B Titre : Recent advances in cytometry Type de document : texte imprimé Mention d'édition : 5th ed. Editeur : Amsterdam : Elsevier/Academic Press Année de publication : 2011 Collection : num. v. 103 Importance : 1 ressource dématérialisée ISBN/ISSN/EAN : 978-0-12-385493-3 Note générale : Revised edition of: Cytometry. 4th ed. c2004
Includes bibliographical references and indexLangues : Anglais Tags : Cytométrie de flux Méthodologie Cytogénétique Index. décimale : Résumé : Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technologyAdvances in applications, Part B. Recent advances in cytometry [texte imprimé] . - 5th ed. . - [S.l. : s.n.], 2011 . - 1 ressource dématérialisée. - (; v. 103) .
ISBN : 978-0-12-385493-3
Revised edition of: Cytometry. 4th ed. c2004
Includes bibliographical references and index
Langues : Anglais
Tags : Cytométrie de flux Méthodologie Cytogénétique Index. décimale : Résumé : Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technologyExemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Laser manipulation of cells and tissues
Titre : Laser manipulation of cells and tissues Type de document : texte imprimé Editeur : Amsterdam : Elsevier Academic Press Année de publication : cop. 2007 Collection : num. v. 82 Importance : 1 ressource dématérialisée ISBN/ISSN/EAN : 978-0-12-370648-5 Note générale : Includes bibliographical references and index Langues : Anglais Tags : Lasers en cytologie Cytologie Technique Cytométrie de flux Index. décimale : Résumé : The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. This book ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one’s own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology.
* Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research
* Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology
* Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchersLaser manipulation of cells and tissues [texte imprimé] . - [S.l. : s.n.], cop. 2007 . - 1 ressource dématérialisée. - (; v. 82) .
ISBN : 978-0-12-370648-5
Includes bibliographical references and index
Langues : Anglais
Tags : Lasers en cytologie Cytologie Technique Cytométrie de flux Index. décimale : Résumé : The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. This book ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one’s own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology.
* Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research
* Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology
* Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchersExemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Part A, Instrumentation, methods. Recent advances in cytometry
Titre de série : Part A, Instrumentation, methods Titre : Recent advances in cytometry Type de document : texte imprimé Mention d'édition : 5th ed. Editeur : Amsterdam : Elsevier/Academic Press Année de publication : 2011 Collection : num. v. 102 Importance : 1 ressource dématérialisée ISBN/ISSN/EAN : 978-0-12-374912-3 Note générale : Revised edition of: Cytometry. 4th ed. c2004
Includes bibliographical references and indexLangues : Anglais Tags : Cytométrie de flux Méthodologie Cytogénétique Index. décimale : Résumé : Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technologyPart A, Instrumentation, methods. Recent advances in cytometry [texte imprimé] . - 5th ed. . - [S.l. : s.n.], 2011 . - 1 ressource dématérialisée. - (; v. 102) .
ISBN : 978-0-12-374912-3
Revised edition of: Cytometry. 4th ed. c2004
Includes bibliographical references and index
Langues : Anglais
Tags : Cytométrie de flux Méthodologie Cytogénétique Index. décimale : Résumé : Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technologyExemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Quantitative imaging in cell biology
Titre : Quantitative imaging in cell biology Type de document : texte imprimé Mention d'édition : 1st. edition Editeur : Amsterdam : Elsevier/AP Année de publication : 2014 Collection : num. volume 123 Importance : 1 ressource dématérialisée (588 pages). ISBN/ISSN/EAN : 978-0-12-420138-5 Note générale : Includes bibliographical references and index Langues : Anglais Tags : Imagerie en biologie Cytométrie de flux Microscopie Index. décimale : Résumé : This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications.
The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
Key Features
Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
Chapters are written by experts in the field
Cutting-edge materialQuantitative imaging in cell biology [texte imprimé] . - 1st. edition . - [S.l. : s.n.], 2014 . - 1 ressource dématérialisée (588 pages).. - (; volume 123) .
ISBN : 978-0-12-420138-5
Includes bibliographical references and index
Langues : Anglais
Tags : Imagerie en biologie Cytométrie de flux Microscopie Index. décimale : Résumé : This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications.
The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
Key Features
Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
Chapters are written by experts in the field
Cutting-edge materialExemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire