Environmental microbiology / Ian T Paulsen

Public ISBD Titre : Environmental microbiology : methods and protocols Type de document : texte imprimé Auteurs : Ian T Paulsen, Editeur scientifique ; Andrew J Holmes, Editeur scientifique Mention d'édition : Second edition Editeur : New York : Humana Press Année de publication : cop. 2014 Autre Editeur : Heidelberg : Springer Collection : num. 1096 Importance : 1 vol. (X-242 p.) Présentation : ill. en noir et en coul., graphs., schémas, tabs., couv. ill. en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-62703-712-9 Langues : Anglais Tags : Life Sciences  Microbiology  Environmental Science and Engineering  Microbiologie de l'environnement  Manuel de laboratoire  Bactéries  isolement et purification  Techniques microbiologiques  Environnement  Microbiologie  Life sciences Index. décimale : Résumé : An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more.Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities.This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes Environmental microbiology : methods and protocols [texte imprimé] / Ian T Paulsen, Editeur scientifique ; Andrew J Holmes, Editeur scientifique  . -  Second edition . - [S.l. : s.n.] : Heidelberg : Springer, cop. 2014 . - 1 vol. (X-242 p.) : ill. en noir et en coul., graphs., schémas, tabs., couv. ill. en coul ; 26 cm. - (; 1096) . ISBN : 978-1-62703-712-9 Langues : Anglais Tags : Life Sciences  Microbiology  Environmental Science and Engineering  Microbiologie de l'environnement  Manuel de laboratoire  Bactéries  isolement et purification  Techniques microbiologiques  Environnement  Microbiologie  Life sciences Index. décimale : Résumé : An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more.Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities.This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes

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Microbial Biofilms / Gianfranco Donelli

Public ISBD Titre : Microbial Biofilms : Methods and Protocols Type de document : document électronique Auteurs : Gianfranco Donelli, Editeur scientifique Editeur : New York : Springer Science + Business Media Année de publication : cop. 2014 Collection : num. 1147 Importance : 1 vol. (XIII-372 p.) Présentation : ill. en noir et en coul., couv. ill. en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-0466-2 Langues : Anglais Tags : Life Sciences  Microbiology  Bacteriology  Bactéries  Adhésivité  Biofilms  Adhérence bactérienne  Life sciences Index. décimale : Résumé : The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms. Microbial Biofilms : Methods and Protocols [document électronique] / Gianfranco Donelli, Editeur scientifique . - [S.l. : s.n.], cop. 2014 . - 1 vol. (XIII-372 p.) : ill. en noir et en coul., couv. ill. en coul ; 26 cm. - (; 1147) . ISBN : 978-1-4939-0466-2 Langues : Anglais Tags : Life Sciences  Microbiology  Bacteriology  Bactéries  Adhésivité  Biofilms  Adhérence bactérienne  Life sciences Index. décimale : Résumé : The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms.

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Quantitative Real-Time PCR / Roberto Biassoni

Public ISBD Titre : Quantitative Real-Time PCR : methods and protocols Type de document : texte imprimé Auteurs : Roberto Biassoni, Editeur scientifique ; Alessandro Raso, Editeur scientifique Editeur : New York, NY : Humana Press Autre Editeur : Heidelberg : Springer Collection : num. 1160 Importance : 1 vol. (XI-231 p.) Présentation : ill. en noir et en coul., graphs, tabs Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-0732-8 Langues : Anglais Tags : Life Sciences  Animal Genetics and Genomics  Human Genetics  PCR (génétique)  Microbiologie  Technique  Réaction de polymérisation en chaine en temps réel  Techniques microbiologiques  Life sciences  Human genetics  Animal genetics Index. décimale : Résumé : Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation Quantitative Real-Time PCR : methods and protocols [texte imprimé] / Roberto Biassoni, Editeur scientifique ; Alessandro Raso, Editeur scientifique . - [S.l. : s.n.] : Heidelberg : Springer, [s.d.] . - 1 vol. (XI-231 p.) : ill. en noir et en coul., graphs, tabs ; 26 cm. - (; 1160) . ISBN : 978-1-4939-0732-8 Langues : Anglais Tags : Life Sciences  Animal Genetics and Genomics  Human Genetics  PCR (génétique)  Microbiologie  Technique  Réaction de polymérisation en chaine en temps réel  Techniques microbiologiques  Life sciences  Human genetics  Animal genetics Index. décimale : Résumé : Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation

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Pseudomonas Methods and Protocols / Alain Filloux

Public ISBD Titre : Pseudomonas Methods and Protocols Type de document : texte imprimé Auteurs : Alain Filloux (1961-..), Editeur scientifique ; Juan-Luis Ramos (1956-..), Editeur scientifique Editeur : New York : Springer Science + Business Media Année de publication : cop. 2014 Collection : num. 1149 Importance : 1 vol. (XVIII-816 p.) Présentation : ill., couv. ill en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-0472-3 Langues : Anglais Tags : Pseudomonas  Microbiologie  Life sciences  Microbiology  Bacteriology Index. décimale : Résumé : In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings. Pseudomonas Methods and Protocols [texte imprimé] / Alain Filloux (1961-..), Editeur scientifique ; Juan-Luis Ramos (1956-..), Editeur scientifique . - [S.l. : s.n.], cop. 2014 . - 1 vol. (XVIII-816 p.) : ill., couv. ill en coul ; 26 cm. - (; 1149) . ISBN : 978-1-4939-0472-3 Langues : Anglais Tags : Pseudomonas  Microbiologie  Life sciences  Microbiology  Bacteriology Index. décimale : Résumé : In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.

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Site-Specific Protein Labeling / Arnaud Gautier

Public ISBD Titre : Site-Specific Protein Labeling : Methods and Protocols Type de document : texte imprimé Auteurs : Arnaud Gautier (1979-..), Editeur scientifique ; Marlon J Hinner, Editeur scientifique Editeur : New York : Humana Press Année de publication : cop. 2015 Collection : num. 1266 Importance : 1 vol. (XII-267 p.) Présentation : ill; en noir et en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-2271-0 Langues : Anglais Tags : Life Sciences  Protein Science  Protein Structure  Protéines  Analyse  Méthodologie  Biologie moléculaire  analyse Index. décimale : Résumé : This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question. Site-Specific Protein Labeling : Methods and Protocols [texte imprimé] / Arnaud Gautier (1979-..), Editeur scientifique ; Marlon J Hinner, Editeur scientifique . - [S.l. : s.n.], cop. 2015 . - 1 vol. (XII-267 p.) : ill; en noir et en coul ; 26 cm. - (; 1266) . ISBN : 978-1-4939-2271-0 Langues : Anglais Tags : Life Sciences  Protein Science  Protein Structure  Protéines  Analyse  Méthodologie  Biologie moléculaire  analyse Index. décimale : Résumé : This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

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