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Humana Press
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Titre : Agrobacterium Protocols Type de document : texte imprimé Mention d'édition : 3rd ed Editeur : New York : Humana Press Année de publication : cop. 2015 Collection : num. 1223 Importance : 1 vol. (XIV-366 p.) Présentation : ill. en noir et en coul., couv. ill Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1694-8 Langues : Anglais Tags : Agrobacterium Techniques bactériologiques Biotechnologie Agrobacterium tumefaciens génétique isolement et purification Végétaux génétiquement modifiés Index. décimale : Résumé : Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Agrobacterium Protocols [texte imprimé] . - 3rd ed . - [S.l. : s.n.], cop. 2015 . - 1 vol. (XIV-366 p.) : ill. en noir et en coul., couv. ill ; 26 cm. - (; 1223) .
ISBN : 978-1-4939-1694-8
Langues : Anglais
Tags : Agrobacterium Techniques bactériologiques Biotechnologie Agrobacterium tumefaciens génétique isolement et purification Végétaux génétiquement modifiés Index. décimale : Résumé : Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Exemplaires
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Titre : Agrobacterium Protocols Type de document : texte imprimé Mention d'édition : 3rd ed Editeur : New York : Humana Press Année de publication : cop. 2015 Collection : num. 1224 Importance : 1 vol. (XVI-378 p.) Présentation : ill. en noir et en coul., couv. ill Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1657-3 Langues : Anglais Tags : Agrobacterium Techniques bactériologiques Biotechnologie Agrobacterium tumefaciens génétique isolement et purification Végétaux génétiquement modifiés Techniques génétiques Index. décimale : Résumé : Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Agrobacterium Protocols [texte imprimé] . - 3rd ed . - [S.l. : s.n.], cop. 2015 . - 1 vol. (XVI-378 p.) : ill. en noir et en coul., couv. ill ; 26 cm. - (; 1224) .
ISBN : 978-1-4939-1657-3
Langues : Anglais
Tags : Agrobacterium Techniques bactériologiques Biotechnologie Agrobacterium tumefaciens génétique isolement et purification Végétaux génétiquement modifiés Techniques génétiques Index. décimale : Résumé : Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Exemplaires
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Titre : Bacterial pangenomics : methods and protocols Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : cop. 2015 Collection : num. 1231 Importance : 1 vol. (x-298 p.) Présentation : ill. en noir et en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1719-8 Langues : Anglais Tags : Génome bactérien Électrophorèse Voies et réseaux métaboliques Techniques de laboratoire clinique Index. décimale : Résumé : Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.Bacterial pangenomics : methods and protocols [texte imprimé] . - [S.l. : s.n.], cop. 2015 . - 1 vol. (x-298 p.) : ill. en noir et en coul ; 26 cm. - (; 1231) .
ISBN : 978-1-4939-1719-8
Langues : Anglais
Tags : Génome bactérien Électrophorèse Voies et réseaux métaboliques Techniques de laboratoire clinique Index. décimale : Résumé : Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.Exemplaires
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Titre : Stem cell protocols Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : cop. 2015 Autre Editeur : Heidelberg : Springer Collection : num. 1235 Importance : 1 vol. (XI- 281 p.) Présentation : ill. en noir et en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1784-6 Langues : Anglais Tags : Cellules souches Cellules endothéliales Système hématopoiétique Ingénierie tissulaire Index. décimale : Résumé : This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.Stem cell protocols [texte imprimé] . - [S.l. : s.n.] : Heidelberg : Springer, cop. 2015 . - 1 vol. (XI- 281 p.) : ill. en noir et en coul ; 26 cm. - (; 1235) .
ISBN : 978-1-4939-1784-6
Langues : Anglais
Tags : Cellules souches Cellules endothéliales Système hématopoiétique Ingénierie tissulaire Index. décimale : Résumé : This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.Exemplaires
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Titre : Advanced protocols in oxidative stress III Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : cop. 2015 Autre Editeur : Springer Collection : num. 1208 Importance : 1 vol. (xiv-477 p.) Présentation : ill Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1440-1 Langues : Anglais Tags : Stress oxydatif physiologie Antioxydants Espèces réactives de l'azote métabolisme Expression des gènes Biostatistiques Index. décimale : Résumé : Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.Advanced protocols in oxidative stress III [texte imprimé] . - [S.l. : s.n.] : [S.l.] : Springer, cop. 2015 . - 1 vol. (xiv-477 p.) : ill ; 26 cm. - (; 1208) .
ISBN : 978-1-4939-1440-1
Langues : Anglais
Tags : Stress oxydatif physiologie Antioxydants Espèces réactives de l'azote métabolisme Expression des gènes Biostatistiques Index. décimale : Résumé : Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.Exemplaires
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