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Stem cell protocols
Titre : Stem cell protocols Type de document : texte imprimé Editeur : New York : Humana Press Année de publication : cop. 2015 Autre Editeur : Heidelberg : Springer Collection : num. 1235 Importance : 1 vol. (XI- 281 p.) Présentation : ill. en noir et en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-1784-6 Langues : Anglais Tags : Cellules souches Cellules endothéliales Système hématopoiétique Ingénierie tissulaire Index. décimale : Résumé : This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.Stem cell protocols [texte imprimé] . - [S.l. : s.n.] : Heidelberg : Springer, cop. 2015 . - 1 vol. (XI- 281 p.) : ill. en noir et en coul ; 26 cm. - (; 1235) .
ISBN : 978-1-4939-1784-6
Langues : Anglais
Tags : Cellules souches Cellules endothéliales Système hématopoiétique Ingénierie tissulaire Index. décimale : Résumé : This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Quantitative Real-Time PCR
Titre : Quantitative Real-Time PCR : methods and protocols Type de document : texte imprimé Editeur : New York, NY : Humana Press Autre Editeur : Heidelberg : Springer Collection : num. 1160 Importance : 1 vol. (XI-231 p.) Présentation : ill. en noir et en coul., graphs, tabs Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-0732-8 Langues : Anglais Tags : Life Sciences Animal Genetics and Genomics Human Genetics PCR (génétique) Microbiologie Technique Réaction de polymérisation en chaine en temps réel Techniques microbiologiques Life sciences Human genetics Animal genetics Index. décimale : Résumé : Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation Quantitative Real-Time PCR : methods and protocols [texte imprimé] . - [S.l. : s.n.] : Heidelberg : Springer, [s.d.] . - 1 vol. (XI-231 p.) : ill. en noir et en coul., graphs, tabs ; 26 cm. - (; 1160) .
ISBN : 978-1-4939-0732-8
Langues : Anglais
Tags : Life Sciences Animal Genetics and Genomics Human Genetics PCR (génétique) Microbiologie Technique Réaction de polymérisation en chaine en temps réel Techniques microbiologiques Life sciences Human genetics Animal genetics Index. décimale : Résumé : Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Yeast protocols
Titre : Yeast protocols Type de document : texte imprimé Mention d'édition : 3rd edition Editeur : New York : Humana Press Année de publication : cop. 2014 Autre Editeur : Heidelberg : Springer Collection : num. 1163 Importance : 1 vol. (X-313 p.) Présentation : ill. en noir et en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-4939-0798-4 Langues : Anglais Tags : Levures physiologie cytologie isolement et purification Levure (agent de fermentation) Physiologie Index. décimale : Résumé : Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.Yeast protocols [texte imprimé] . - 3rd edition . - [S.l. : s.n.] : Heidelberg : Springer, cop. 2014 . - 1 vol. (X-313 p.) : ill. en noir et en coul ; 26 cm. - (; 1163) .
ISBN : 978-1-4939-0798-4
Langues : Anglais
Tags : Levures physiologie cytologie isolement et purification Levure (agent de fermentation) Physiologie Index. décimale : Résumé : Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Environmental microbiology
Titre : Environmental microbiology : methods and protocols Type de document : texte imprimé Mention d'édition : Second edition Editeur : New York : Humana Press Année de publication : cop. 2014 Autre Editeur : Heidelberg : Springer Collection : num. 1096 Importance : 1 vol. (X-242 p.) Présentation : ill. en noir et en coul., graphs., schémas, tabs., couv. ill. en coul Format : 26 cm ISBN/ISSN/EAN : 978-1-62703-712-9 Langues : Anglais Tags : Life Sciences Microbiology Environmental Science and Engineering Microbiologie de l'environnement Manuel de laboratoire Bactéries isolement et purification Techniques microbiologiques Environnement Microbiologie Life sciences Index. décimale : Résumé : An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more.Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities.This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes Environmental microbiology : methods and protocols [texte imprimé] . - Second edition . - [S.l. : s.n.] : Heidelberg : Springer, cop. 2014 . - 1 vol. (X-242 p.) : ill. en noir et en coul., graphs., schémas, tabs., couv. ill. en coul ; 26 cm. - (; 1096) .
ISBN : 978-1-62703-712-9
Langues : Anglais
Tags : Life Sciences Microbiology Environmental Science and Engineering Microbiologie de l'environnement Manuel de laboratoire Bactéries isolement et purification Techniques microbiologiques Environnement Microbiologie Life sciences Index. décimale : Résumé : An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more.Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities.This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Physique des écoulements continus
Titre : Physique des écoulements continus Type de document : texte imprimé Editeur : Heidelberg : Springer Année de publication : cop. 2013 Collection : num. 74 Importance : 1 vol. (XII-323 p.) Présentation : fig Format : 24 cm ISBN/ISSN/EAN : 978-3-642-39509-3 Langues : Français Tags : Mathematics Mechanics Fluides, Mécanique des Applications (mathématiques) Index. décimale : Résumé : La mecanique des fluides est abordee sous deux points de vue, physique et mathematique. Les bases de la mecanique des milieux continus sont d'abord presentees en detail en precisant les hypotheses et approximations qui conduisent aux lois de conservation. Les outils d'analyse des equations generales, etude en ordres de grandeurs, analyse adimensionnelle et similitude, permettent ensuite d'introduire les approximations de fluide parfait d'Euler, de fluides visqueux en regime de Stokes et de la couche limite de Prandtl. Les notions de stabilite des ecoulements, de turbulence et les ecoulements compressibles sont ensuite presentes. Les ecoulements multiphysiques ouvrent des perspectives pour la recherche et le traitement d'applications complexes. In this book, fluid mechanics is addressed from both physical and mathematical perspectives. As a first step, the basic theory of continuum mechanics is detailed, pointing out the different assumptions and approximations that lead to conservation laws. As a second step, scaling studies, dimensional analysis and similitude of the general equations help to introduce the inviscid Euler fluid approximation, the Stokes approximation of viscous flow and Prandtl's boundary layer approximation. Lastly, the concepts of stability, turbulence and compressible flows are presented. Multiphysics approach of flows will provide readers with deeper insights into basic and applied research on complex processing. Physique des écoulements continus [texte imprimé] . - [S.l. : s.n.], cop. 2013 . - 1 vol. (XII-323 p.) : fig ; 24 cm. - (; 74) .
ISBN : 978-3-642-39509-3
Langues : Français
Tags : Mathematics Mechanics Fluides, Mécanique des Applications (mathématiques) Index. décimale : Résumé : La mecanique des fluides est abordee sous deux points de vue, physique et mathematique. Les bases de la mecanique des milieux continus sont d'abord presentees en detail en precisant les hypotheses et approximations qui conduisent aux lois de conservation. Les outils d'analyse des equations generales, etude en ordres de grandeurs, analyse adimensionnelle et similitude, permettent ensuite d'introduire les approximations de fluide parfait d'Euler, de fluides visqueux en regime de Stokes et de la couche limite de Prandtl. Les notions de stabilite des ecoulements, de turbulence et les ecoulements compressibles sont ensuite presentes. Les ecoulements multiphysiques ouvrent des perspectives pour la recherche et le traitement d'applications complexes. In this book, fluid mechanics is addressed from both physical and mathematical perspectives. As a first step, the basic theory of continuum mechanics is detailed, pointing out the different assumptions and approximations that lead to conservation laws. As a second step, scaling studies, dimensional analysis and similitude of the general equations help to introduce the inviscid Euler fluid approximation, the Stokes approximation of viscous flow and Prandtl's boundary layer approximation. Lastly, the concepts of stability, turbulence and compressible flows are presented. Multiphysics approach of flows will provide readers with deeper insights into basic and applied research on complex processing. Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire Plant Cell and Tissue Culture - A Tool in Biotechnology
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